In the Building: Labs

Contact


  • Peter Burke
  • Office Location: 1309 Calit2 Building

Bio-Nano Lab

This lab is equipped and designed for integration of research themes in the area of nano-systems biology, bio-chemistry and nanotechnology. Approximately 836 square footage lab space is equipped with several wet benches and countertop storage racks, two metal storage cabinets (for flammable and corrosive materials) with attached exhaust line, two fume hoods with chemical and heat resistant interior surface and color coded remotely operated taps for vacuum, compressed air, nitrogen, cold water and DI water along with under the hood metal storage cabinets for acid and chemical waste storage. Ultrapure DI water with a resistivity of upto 18.2 M?/cm and with total organic carbon (TOC) level as low as < 1ppb is available. In addition, a Lab safe refrigerator (+4 ºC, VWR Model # GDM-23) with see through glass door and internal power outlets and a Lab safe Freezer (-20 ºC, VWR Model #SCBMF 2020) are in operation for the storage of biological and chemical samples. The current list of equipments that we have brought to the lab is as below:

1.Incubator/Shaker - New Brunswick Scientific Classic Series (Model C24)
2.Isotemp Incubator - Fisher Scientific
3.Autoclave - Harvey Sterile Max
4.Small Oven - Boekel Scientific
5.Centrifuge - Eppendorf 5417R
6.Sonicator - VWR B1500A R
7.Mini Shaker - Fisher Scientific
8.Water Bath Heater, Mixer, Shaker - VWR
9.Weighing Balance - Sartorious - GD 503 - NTEP
10.Microwave - GE
11.Gel Imaging System - Kodak Gel Logic 200
12. Desiccators
13. Syringe pump
14. Two hot plates
15.Miscellaneous chemicals

An inverted fluorescent microscope and a patch-clamp systems including a micromanipulator on a vibration isolation table are currently being installed.

These facilities enable research at the intersection of biology, nanotechnology, information technology, and medicine in an integrated lab, which is not possible in traditional dry labs or clean rooms, and also not possible in traditional medical and biology labs. The ability to demonstrate and test nanodevices as prototype probes of cellular and subcellar function is a unique aspect of this lab, integrated with the device fabrication facilities (INRF, BION) and nano- and materials characterization facilities (Zeiss lab) under the umbrella organization of CalIT2.

Some examples of enabled research includes wet-lab projects include cell sorting and analysis, DNA sequencing, the formation of artificial cells, nanotube chemistry for printed biosensors, electrochemical based apoptosis assays, reactive free radical chemistry of DNA damage, neural probes at the sub-cellular level, and bio-energetic studies of metabolism at the fundamental single protein level, and various microfluidic based lab on a chip systems. These projects generally will enable a better understanding of (and eventually improved diagnosis and treatment of) a variety of diseases, including, for example cancer, diabetes, and the aging process itself.

Some currently funded projects include support from sources such as the National Cancer Institute (of the NIH) and the Army Research Office.